A cryostat is a specialized microtome housed within a refrigerated chamber, designed for cutting extremely thin sections of fresh or fixed frozen tissue. This instrument is crucial for rapid tissue preparation, especially for applications requiring the preservation of enzyme activity and antigenicity, such as immunohistochemistry, immunofluorescence, and in situ hybridization. The chamber maintains cryogenic temperatures, typically ranging from 0°C to -35°C, with cryobars reaching -55°C, to ensure tissue integrity and prevent ice crystal formation. Cryostats allow for section thickness adjustments, commonly from 1 to 60 micrometers, enabling detailed microscopic examination. They are widely used in research and clinical settings for quick diagnostic evaluations, including intraoperative consultations.