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Protocol Cortex

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Their protocols keep getting better.

Every improvement proposed, reviewed, and merged. The knowledge compounds.

Describe a protocol. AI writes the draft. Free to start.

Sarah proposes changes to your lab template. You review the diff and decide.

Western Blot - StandardSubmitted by Sarah Chen for your review
Analysis completeMerges into your lab template

Summary Substitutes BSA with non-fat milk for improved blocking. Extends incubation and wash cycles. 3 literature sources.

3 units·10 changes·Low risk·Ready to apply·Literature-backed reagent substitution with linked incubation changes
Change Units
Current Template
Proposed Changes
Reagent Substitution
2
Linked3 sources

Why linked: BSA-to-milk swap requires adjusted incubation for optimal blocking.

Chen 2022Park 2021BioProtocol
Risk: LOWAccepted
Phase 2Blocking
60 min
1
Block membrane with 5% BSA in TBS-T
2
Incubate for 1 hour at room temperature
3
Do not let membrane dry during incubation
Phase 2Blocking
120 min
1
Block membrane with 5% non-fat milk in TBS-T
2
Incubate for 2 hours at room temperature
3
Do not let membrane dry during incubation
Chen, R. et al. (2022)J. Immunological MethodsVerified847 citations

Key finding: 40% lower background signal with non-fat milk vs BSA across 6 antibody-antigen pairs.

View
Wash Optimization
4
Linked2 sources
Accepted
Phase 3Washing
15 min
1
Wash 3x with TBS-T, 5 min each
2
Rock membrane at 15 rpm on platform shaker
3
Decant buffer between washes without touching membrane
4
Ensure complete removal of blocking agent
5
Rinse once with TBST before next phase
6
Blot excess liquid from membrane edge on filter paper
Phase 3Washing
50 min
1
Wash 5x with TBS-T, 10 min each
2
Rock membrane at 25 rpm on platform shaker
3
Decant buffer between washes without touching membrane
4
Ensure complete removal of blocking agent
5
Rinse twice with TBST before next phase
6
Blot excess liquid from membrane edge on filter paper
Temperature Adjustment
3
Independent1 source
Click to review
Phase 4Primary Antibody
12 hours
1
Incubate at 4°C overnight
2
Dilute primary 1:1000 in 5% BSA/TBST
3
Use minimal volume to cover membrane evenly
4
Protect from light
5
Do not add sodium azide to antibody buffer
6
Store plate wrapped in foil at 4°C until imaging
Phase 4Primary Antibody
2 hours
1
Incubate at room temperature for 2 hours
2
Dilute primary 1:2000 in 5% milk/TBST
3
Use minimal volume to cover membrane evenly
4
Protect from light
5
Do not add sodium azide to antibody buffer
6
Discard primary antibody after single use

Built for labs where protocols are living science, not static documents

Propose and Merge

Researchers propose improvements to lab templates. PIs review the diff. The best ideas get merged. Like version control, but for science.

AI-Powered Review

Every proposal analyzed in three phases: structural diff, AI-generated insights, and literature citations that validate the change.

Inventory on Autopilot

Every protocol run deducts reagents automatically. Running low triggers a reorder. You never write a purchase request again.

Full Version History

Every optimization tracked. Every version recoverable. Complete audit trail of who changed what, when, and why.

One protocol run. Four systems updated.

Protocol Run: Western Blot v3.2Complete

Reagents deducted. Reorders placed.

BSA, TBS-T, non-fat milk. Every material listed in the protocol is deducted the moment the run completes. Drop below threshold? The reorder goes out before anyone notices.

-250ml BSA

Reagents deducted. Reorders placed.

BSA, TBS-T, non-fat milk. Every material listed in the protocol is deducted the moment the run completes. Drop below threshold? The reorder goes out before anyone notices.

-250ml BSA

Next slot booked. Before you ask.

The protocol needs 2 hours on the plate reader tomorrow. The system checks availability and holds the slot. Your grad student just confirms.

2h reserved

Next slot booked. Before you ask.

The protocol needs 2 hours on the plate reader tomorrow. The system checks availability and holds the slot. Your grad student just confirms.

2h reserved

Every hour tracked. Every sample logged.

Equipment hours, human hours, sample prep time. All captured per-project as the protocol runs. Not reconstructed from memory at billing time.

3.5h tracked

Every hour tracked. Every sample logged.

Equipment hours, human hours, sample prep time. All captured per-project as the protocol runs. Not reconstructed from memory at billing time.

3.5h tracked

End of month. One click.

Every protocol run tracked every resource. Generating the invoice is not a research project of its own. One button. Accurate to the reagent.

Invoice ready

End of month. One click.

Every protocol run tracked every resource. Generating the invoice is not a research project of its own. One button. Accurate to the reagent.

Invoice ready

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Protocol Cortex answers all of these. By making the protocol the source of truth.

Your lab is already running protocols. Now make them run your lab.

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Describe a protocol. AI writes the draft. Free to start.