The Fluidigm Hyperion Imaging System is a complementary technology to flow-based mass cytometry, enabling spatial analysis of tissue samples with metal-conjugated antibodies. It is the world's first and only commercially available metal-tagged-antibody-directed Imaging Mass Cytometry (IMC) platform. Hyperion achieves single-cell resolution by scanning tissue sections with a small-spot-size pulsed laser (1 µm²), vaporizing 1-micron spots and analyzing them using mass cytometry. This allows for the mapping of target proteins throughout the tissue section, preserving spatial localization and enabling the digital reconstruction of tissues. Researchers can interrogate tissues with over 40 (up to 50) parameters simultaneously, providing rich datasets that combine protein expression and spatial localization within an intact tissue sample. It is compatible with fixed tissue sections, cells deposited on glass slides, formalin-fixed paraffin-embedded (FFPE), and snap-frozen tissues, making it suitable for analyzing small and precious archived pathology samples.