This setup provides tools for quantitative analysis of three-dimensional biological structures from two-dimensional microscopic images. It is used for unbiased numerical estimation of parameters such as volume, length, surface area, and population size of cells, organelles, and tissues. The workstation combines advanced microscopy with specialized software for image acquisition, processing, and statistical analysis. It is crucial for studies requiring precise quantification of morphological changes, enabling researchers to obtain accurate and reproducible data from biological samples, particularly in histology and neuroscience research.