The Douglas Molecular and Cellular Microscopy Platform (MCMP) is an advanced optical microscopy core facility established in 2015 at the Douglas Research Centre. Its primary purpose is to provide researchers with access to cutting-edge fluorescence microscopy techniques essential for neuroscience research, assisting them in the planning, design, execution, and analysis of their microscopy experiments. As an Olympus Discovery Center, the MCMP offers access to a comprehensive suite of state-of-the-art equipment. This includes several wide-field microscopes integrated with MicroBrightField software analysis systems, an Olympus FV1200 upright confocal microscope, a high-throughput Olympus VS120 slide scanner, and an ImageXpress High-Content Screening Microscope. A standout feature is a custom-built Olympus FVMPE-RS advanced multi-photon microscope, specifically designed for high-speed, millisecond imaging in deep tissue, making it ideal for live-animal calcium imaging and neuronal stimulation with optical fibers. This unique multiphoton system comprises two distinct microscopes sharing a single InSight DeepSee pulsed IR laser, one optimized for live animal imaging and the other tailored for optogenetics and electrophysiology in live brain slices. It also facilitates exceptionally deep imaging (up to 8mm) of clarified specimens (CLARITY) using specialized Olympus objectives. The platform supports a diverse range of imaging applications, from fundamental neuron reconstruction to complex, high-speed, deep-tissue optogenetic experiments conducted in brain slices or live animals. It is an indispensable resource for neuroscience research, enabling detailed visualization of cellular structures and molecular processes, and is crucial for analyzing brain imaging data derived from functional and structural neuroimaging techniques. Beyond its advanced instrumentation, the MCMP provides personalized one-on-one support and comprehensive training for fluorescence microscopy experiments and image analysis. The platform utilizes OMERO for efficient image management, allowing direct import of OIR-format multiphoton images and seamless integration with OMERO.figure for advanced analysis. Additional services include access to an image server, specialized 3D imaging services, and high-volume slide scanning.