MDAMB-231 is a widely used human breast cancer cell line in cancer research. These cells are highly aggressive and metastatic, making them valuable for studying cancer progression and therapeutic interventions. They are derived from a patient with metastatic breast adenocarcinoma., These cells are commonly used in in vitro and in vivo experiments to investigate tumor growth, invasion, and metastasis. The cells are adherent and require specific growth conditions, including DMEM supplemented with 10% fetal bovine serum (FBS) and antibiotics. Passage number and source information are critical for reproducibility.