Leica AF Lite is a free-to-download software application developed by Leica Microsystems that allows users to view .lif image files, a proprietary format commonly generated by Leica microscopes. This software is essential for researchers who need to access and review microscopy data acquired on Leica systems without requiring the full Leica Application Suite X (LAS X) software. The software enables users to open and navigate through multi-dimensional .lif datasets, including time-lapse, Z-stack, and multi-channel images. It also provides functionality to save these images in more open and widely compatible image formats, facilitating data sharing and analysis with other software platforms. Leica AF Lite is a convenient tool for basic image viewing and conversion, making microscopy data more accessible for collaborators and for integration into various image analysis workflows. It is particularly useful for quick data checks and for users who do not require the advanced acquisition and analysis features of the full LAS X suite. This digital good is provided by Leica Microsystems to support the usability of their imaging systems and data. It is a lightweight solution for managing and converting .lif files.