Zeiss LSM880 ElyraPS1 laser scanning confocal and super-resolution microscope (SIM and PALM / dSTORM)

Specifications

Microscopy stand: Inverted Axio Observer Z1

Software: ZEN2012

Detector for confocal: 34 channel spectral R/FL detectors

(32 GaAsp detectors array)

Cameras: Andor EMCCD camera iXon 885 and EMCCD iXon DU 897

Confocal Lasers: 405 nm, 458, 488 and 514 nm multiline, 561 nm and 633 nm

SIM/PALM lasers: 405 nm, 488nm, 561 nm and 640 nm

Objectives:

• EC Plan-Neofluar 10x/0.30 WD=5.2
• Plan-Apochromat 20x/0.8 WD=0.55
• Plan-APO 40x/1.4 Oil DIC (UV) VIS-IR
• Plan-Apochromat 63x/1.40 Oil DIC f/ELYRA
• Plan-APO 100x/1.46 Oil DIC VIS

Applications

Laser Scanning Confocal

Total Internal Reflection Fluorescence (TIRF)

Super-Resolution:

Structured Illumination (SIM)

Photo Activation Localization (PALM)

Stochastic Optical Reconstruction (dSTORM)