Quantstudio 3D Digital PCR

The QuantStudio 3D Digital PCR System is a digital PCR system designed for the absolute quantification of nucleic acids using chip-based technology. It extends beyond the limits of traditional real-time PCR, offering unparalleled precision, sensitivity, and accuracy for demanding applications.

The system features a simple load-and-go workflow, significantly minimizing hands-on steps and sample manipulation. The process involves loading the reaction mix onto a uniquely tagged chip, sealing it, amplifying on a compatible thermal cycler, and then reading the target concentration in less than a minute on the QuantStudio 3D Digital PCR Instrument. This sealed chip technology ensures samples and amplification products remain contained, reducing the likelihood of cross-contamination and amplicon release into the lab environment.

At its core, the system utilizes a high-density nanofluidic chip manufactured from a silicon substrate, etched with 20,000 consistently sized nanoscale reaction wells. This design enables more controlled sample loading and less reaction dropout compared to droplet-based approaches, ensuring higher precision for absolute quantification. Each chip is sufficient for one duplexed sample, allowing for flexible experimental design without the need to batch samples.