The Inverted Nikon Eclipse Ti2-E is a highly flexible spinning disc confocal microscope equipped with a CrestX-Light V3 spinning disc and dual cameras, offering a large 25mm field of view. This fully motorized, research-grade inverted microscope is engineered for advanced fluorescence and live-cell imaging, supporting high-speed multidimensional imaging. It is ideal for intravital imaging of live tissues (e.g., liver, spleen, intestine) and fast in vitro live cell imaging. The system includes a heated stage insert for intravital imaging and a CO2 incubator with humidity control for in vitro live cell imaging. It supports Nikon's Perfect Focus System (PFS) for maintaining sharp focus during long-term time-lapse and Z-stack imaging, making it suitable for high-throughput phenotypic screenings and high-resolution time-lapse microscopy.