Olympus FV1000MPE multiphoton and laser scanning confocal microscope

Specifications

Microscopy stand: Upright BX61WI microscope with motorized XY stage and portal frame for in vivo imaging

Software: Fluoview Advanced Software

Detector:

• Four (4) channel non-descanned MPE detection
• Three (3) channel visible confocal detection with two (2) of the detectors being spectral
• One (1) transmitted light detector for visible laser DIC

Lasers:

• 405 nm, 440 nm, 488 nm, 514 nm, 561 nm and 635 nm
• Mai Tai HP DeepSee laser (tunable IR laser from 690-1040 nm)
• Dual output enables use of all laser lines for imaging and photo-manipulation
• Live cell module: CO2 and temperature controller

Objective:

• Plan-Neofluar 20x LD

Applications

Multi-fluorescence with DIC

3D examinations (Z-stacks)

Multidimensional acquisition (tile scan, time lapse)

Confocal imaging

Intravital imaging

Live cell imaging

FRET

Photo-manipulation (FRAP, uncaging, etc.)