LLS 7 - Lattice Lightsheet

Long-Term Volumetric Imaging of Living Cells.

Features:

• Environment control including temperature, CO2 and humidity.
• Multi-dimensional imaging (time, position, tiles): combination of multidimensions possible.
• Multi-color images.

Light Sources:

• LED (white & red) for transmitted light
• Laser (488nm, 561nm, 640nm) for reflected light and epi-fluorescence

Detectors:

• 2 cameras: Hamamatsu ORCA-Fusion sCMOS

Objectives:

• Illumination: 13.3 x NA 0.44
• Detection: 44.83 x NA 1.0

Light Sheets available (length x thickness): Beam shaping by cylinder lens and spatial light modulator

• 15µm x 550nm (with side lobes)
• 15µm x 650nm
• 30µm x 700nm (with side lobes)
• 30µm x 1000nm
• 100µm x 1400nm (with side lobes)
• 100µm x 1800nm

Location:

• Located in Bellini Building, room 162

Initial training sessions usually last about 3 hours and include the hourly microscope fee plus the technical support fee.

IMPORTANT: ALL training and technical support is by appointment ONLY and will depend on the availability of the ABIF staff.