This integrated system combines an upright Nikon Yokogawa CSU-X1 spinning disc confocal with a Nikon AX-R point-scanning confocal, primarily designed for advanced intravital microscopy. The CSU-X1 spinning disk is known for its fast imaging capabilities (up to 2000 Hz disk speed) and low phototoxicity, making it excellent for live cell and organism applications. The Nikon AX-R component provides high-resolution point-scanning capabilities, including a large field of view and high-speed resonant scanning. The system is optimized for fast and deep imaging into live tissues such as lungs, breast, flank, skin, and brain, and also supports imaging of cleared organs and tissue sections. It is equipped with multiple laser lines (e.g., 405nm, 488nm, 561nm, 640nm, and 730nm) and dual sCMOS cameras for simultaneous two-channel imaging.