Nuclear Magnetic Resonance (NMR) spectroscopy at 800 MHz provides the highest available sensitivity and resolution, allowing for the most detailed structural and quantitative analysis of metabolites. Equipped with cryogenically cooled probes for improved sensitivity, it is a powerful non-destructive technique for non-targeted metabolomics and metabolic flux analysis. It detects metabolites based on the magnetic properties of atomic nuclei, enabling in-depth analysis of complex biological samples, crucial for identifying and quantifying a wide range of compounds and studying intricate metabolic pathways and disease mechanisms.