The 10x Genomics Xenium platform offers an advanced, end-to-end solution for high-throughput spatial gene expression profiling at subcellular resolution. This powerful tool allows researchers to map and quantify up to 5,000 genes within their spatial context, providing insights into cellular functions and interactions. The platform is compatible with both fresh frozen (FF) and formalin-fixed, paraffin-embedded (FFPE) tissues. Xenium's unique padlock probe chemistry ensures high specificity and sensitivity, enabling precise localization of RNA molecules. It supports future capabilities for multiplexed protein detection, allowing simultaneous RNA and protein analysis. The non-destructive workflow retains tissue morphology for subsequent staining, such as H&E or immunofluorescence. The Xenium Analyzer automates the entire process from sample preparation to onboard data analysis, delivering high-resolution images and detailed gene expression maps viewable with Xenium Explorer. It offers class-leading speed and throughput, analyzing large tissue areas (e.g., 472 mm²) in a short timeframe (e.g., 5,000 genes in ≤6 days).