This resource provides cryopreserved vials containing 1 million viable primary Human Bronchial Epithelial (HBE) cells. These cells are considered the 'gold standard' for in vitro studies due to their physiological relevance, making them an invaluable starting material for a wide range of respiratory research applications. They are isolated from human bronchi and are designed for researchers to initiate and expand their own cell cultures. Each vial contains 1 million cells, cryopreserved to maintain viability and functionality upon thawing. The cells are sourced from the McGill Primary Airway Cell Biobank (PACB), a non-profit facility that ensures standardized, high-quality primary airway cells from both normal and diseased donors. The PACB performs extensive quality control, including routine culture on porous supports and histological examination after 4 weeks in Air-Liquid Interface (ALI) to assess differentiation, and assay of CFTR function in Ussing chambers. Primary HBE cells are widely used for investigating airway physiology and pathophysiology, cell biology, inflammation, and mucosal immunity. They serve as critical models for translational research aimed at developing new therapeutics, drug discovery, disease modeling (e.g., cystic fibrosis, COPD, asthma), toxicity studies of inhaled substances, and studies of respiratory infections. Their ability to retain in vivo-like properties and differentiate into various cell types found in the human airway makes them superior to immortalized cell lines for many applications. The use of these primary cells offers significant benefits, including a more accurate representation of human biology compared to cell lines, the availability of cells from diverse genetic backgrounds, and the assurance of high quality and standardization provided by a certified biobank. This resource enables researchers to conduct more physiologically relevant experiments, leading to more reliable and translatable research outcomes.