The Primary Airway Cell Biobank (PACB) at McGill University offers a comprehensive service for researchers seeking high-quality human primary airway cells and specimens. As the first of its kind in Canada, this non-profit facility provides standardized, high-quality primary airway cells from both normal donors and a wide variety of donors with airway diseases, including Cystic Fibrosis (CF), Chronic Obstructive Pulmonary Disease (COPD), asthma, chronic bronchitis, and emphysema. The PACB is a critical resource for studies in respiratory biology, disease mechanisms, and drug discovery, providing physiologically relevant human models that are considered the 'gold standard' for preclinical development. The PACB maintains stringent quality control measures for all its offerings. Cells and tissues undergo rigorous testing to ensure they are free from mycoplasma, bacteria, yeast, fungi, HIV-1,2, Hepatitis A, B, and C, Hantavirus, and HTLV-1,2. Verification processes include assessing cell purity, viability, and the capacity for differentiation into pseudostratified mucociliary epithelium. For specific applications, such as air-liquid interface studies, the biobank guarantees the differentiation of primary Human Bronchial Epithelial (HBE) cells, marked by cilia formation, mucin production, and stable transepithelial electrical resistance (TEER) levels. Transport function is also validated using Ussing chamber techniques, and CF genotypes are confirmed for relevant samples. The service supports a wide array of research applications, from basic science investigations into airway physiology to translational studies aimed at developing new therapies for respiratory diseases. By providing well-characterized human samples, the PACB enables researchers to overcome limitations associated with animal models and immortalized cell lines, leading to more accurate and clinically relevant findings. The availability of detailed donor information, including age, sex, BMI, ethnicity, diagnosis, medication, serology, FEV1, and specific CF-related data (mutation, antibiogram, CF-associated diabetes, pancreatic insufficiency), further enhances the utility of these resources for stratified research. The PACB also offers specialized cell culture media, such as BEGM and ALI media, and provides personalized technical support. The PACB offers a broad spectrum of specimens. Bronchial specimens include Human Bronchial Epithelial (HBE) cells, Human Tracheal Gland (HTG) cells, airway fibroblasts, airway smooth muscle cells, and sputum. Other available specimens are nasal brushes, nasal polyps, and nasal internal turbinate. The tissue bank provides various airway tissues such as trachea, superior bronchus, inferior bronchus, smooth muscle, parenchyma (alveolar tissue), and endothelium, available as fixed tissue sections in paraffin blocks or OCT, or as snapfrosts of fresh tissues. Additionally, polarized HBE and nasal cells on porous filters, and cytospins of nasal brushes and HBE cells are provided, catering to diverse experimental needs. The biobank also offers transepithelial electrophysiological measurements on a fee-for-service basis for drug discovery projects.