Human nasal brush cytospin samples are prepared from cells collected via nasal brushing, which are then processed using a cytocentrifuge to deposit them onto a microscope slide. This technique provides a monolayer of cells, primarily human nasal epithelial (HNE) cells, suitable for morphological analysis, immunocytochemistry, and molecular studies. Cytospins allow for detailed examination of cellular characteristics, including ciliary structure and function, and the presence of inflammatory cells. They are a rapid and effective method for diagnostic purposes and research into various upper airway diseases, offering insights into cellular pathology and biomarker expression.