Rederivation, line recovery or IVF
Rederivation, line recovery or IVF from live animals or frozen gametes (sperm or embryos) held at MICAM, at the GCI, or from an external source. Used to obtain a mouse line from cryopreserved gametes, pups for lines in which mating is difficult or no longer possible, cohorts for your experiments, or a new health status for an infected colony.
What it takes
| Embryos implanted | About 40 when all share one genotype, about 60 for a mixture. For cohorts, every embryo from the IVF is implanted at once. |
|---|---|
| Males | At least 2 live males aged 3 to 8 months and proven breeders, or 2 straws of frozen sperm |
| Females | At least 5 live females aged 4 to 5 weeks |
| Wild-type strains at MICAM | C57BL/6J, C57BL/6N, FVB and CD-1 |
| Before you order | An approved animal protocol naming the line. Contact us before arranging any shipping or placing any orders; for an external source we advise on the purchase and coordinate transport to the platform. |
A super-ovulated female typically yields 10 to 20 embryos, and multiple IVF attempts may be required, so make sure that multiple males, females or frozen samples are available. When purchasing cryopreserved sperm, ask for the thawing protocol. The procedure is terminal; no sample can be sent back.
Request this service
McGill Integrated Core for Animal Modeling (MICAM) replies with scope and price.
Your details
Nothing is booked or charged until you accept a quote.
How it goes
You ask
Say what you need, in your words.
They scope it
The facility confirms it can be done and may ask for a detail or two.
You accept a quote
Price and timing, agreed before any work starts.
Results come back
Files arrive in your requests, with a note from the facility.
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