What researchers can do
High-speed light-sheet imaging of large cleared samples, up to 40 x 75 mm.
The SmartSPIM is a light-sheet microscope for high-speed volumetric imaging of large, optically cleared samples. An axially swept light sheet keeps axial resolution uniform across the field of view, with compensation for refractive-index mismatch and chromatic focal shifts.
It takes fixed samples from a few millimeters up to 40 x 75 mm and works with many clearing methods: EasyIndex imaging oil (RI 1.52) for SHIELD and CUBIC, and ethyl cinnamate (RI 1.56) for iDISCO and related methods. A second computer processes data while the first acquires.
- High-speed volumetric light-sheet imaging
- High-sensitivity camera with low photobleaching
- Axially swept light sheet for uniform axial resolution
- Simultaneous acquisition and processing
Papers with data from this system acknowledge the Sandler Program for Breakthrough Biomedical Research, partially funded by the Sandler Foundation. Room 472A, Weill Neurosciences Building.
UCSF Innovation Core (Advanced Light Microscopy)
Research lab focused on advancing scientific knowledge and innovation.
CM
Caroline Mrejen
UCSF Innovation Core (Advanced Light Microscopy) · UCSFWhat researchers can do
High-speed light-sheet imaging of large cleared samples, up to 40 x 75 mm.
The SmartSPIM is a light-sheet microscope for high-speed volumetric imaging of large, optically cleared samples. An axially swept light sheet keeps axial resolution uniform across the field of view, with compensation for refractive-index mismatch and chromatic focal shifts.
It takes fixed samples from a few millimeters up to 40 x 75 mm and works with many clearing methods: EasyIndex imaging oil (RI 1.52) for SHIELD and CUBIC, and ethyl cinnamate (RI 1.56) for iDISCO and related methods. A second computer processes data while the first acquires.
- High-speed volumetric light-sheet imaging
- High-sensitivity camera with low photobleaching
- Axially swept light sheet for uniform axial resolution
- Simultaneous acquisition and processing
Papers with data from this system acknowledge the Sandler Program for Breakthrough Biomedical Research, partially funded by the Sandler Foundation. Room 472A, Weill Neurosciences Building.
UCSF Innovation Core (Advanced Light Microscopy)
Research lab focused on advancing scientific knowledge and innovation.
CM
Caroline Mrejen
UCSF Innovation Core (Advanced Light Microscopy) · UCSFYou might also like
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