Stellaris 8 Confocal Microscope

DMi8 motorized and automated inverted microscope stand

STED microscopy and lifetime‐based imaging with tauSense and FALCON (TCSPC‐based FLIM)

Features:

• 4D Imaging, multi-color, z-stack, timelapse
• DIC
• Navigator mode for easy overview and tiling
• LIGHTNING module for enhanced resolution
• STED
• FLIM
• Excitation and emission lambda scans to characterize sample or for spectral unmixing
• Stage‐top incubation system

Light Sources:

• LED3 white light source for epi-fluorescence visualization
• 405nm laser
• Tunable White Light Laser (WLL) with 350 laser lines 440‐790nm (pulsed with 80MHz repetition rate)
• 775nm depletion laser for 3D STED microscopy

Detectors:

• 3 HyD S detectors
• 2 HyD X detectors

Objectives:

PL APO 10x/0.40 CS2

• FWD: 2.56mm
• Coverglass: 0.17mm
• Immersion: dry

PL APO 20x/0.75 CS2

• FWD: 0.62mm
• Coverglass: 0.17mm
• Immersion: dry

PL APO 93x/1.30 GLY motCORR STED WHITE

• FWD: 0.3mm
• Coverglass: 0.14‐0.17mm
• Motorized correction collar (motCORR)
• Immersion: Glycerol

PL APO 100x/1.40 oil STED WHITE

• FWD: 0.13mm
• Coverglass: 0.17mm
• Immersion: oil

Fluorescence Cubes:

15525338 Filter cube LED 405

• Excitation: 405/60
• Emission: 470/40

15525314 Filter Cube GFP

• Excitation: BP 470/40
• Emission: BP 525/50

15525303 Filter Cube RHOD LP

• Excitation: BP 540/45
• Emission: LP 590

Location:

• Located in Bellini Building, Room 151.

Initial training sessions usually last about 3 hours and include the hourly microscope fee plus the technical support fee.

IMPORTANT: ALL training and technical support is by appointment ONLY and will depend on the availability of the ABIF staff.