Stellaris 8 Confocal Microscope
DMi8 motorized and automated inverted microscope stand
STED microscopy and lifetime‐based imaging with tauSense and FALCON (TCSPC‐based FLIM)
Features:
- 4D Imaging, multi-color, z-stack, timelapse
- DIC
- Navigator mode for easy overview and tiling
- LIGHTNING module for enhanced resolution
- STED
- FLIM
- Excitation and emission lambda scans to characterize sample or for spectral unmixing
- Stage‐top incubation system
Light Sources:
- LED3 white light source for epi-fluorescence visualization
- 405nm laser
- Tunable White Light Laser (WLL) with 350 laser lines 440‐790nm (pulsed with 80MHz repetition rate)
- 775nm depletion laser for 3D STED microscopy
Detectors:
- 3 HyD S detectors
- 2 HyD X detectors
Objectives:
PL APO 10x/0.40 CS2
- FWD: 2.56mm
- Coverglass: 0.17mm
- Immersion: dry
PL APO 20x/0.75 CS2
- FWD: 0.62mm
- Coverglass: 0.17mm
- Immersion: dry
PL APO 93x/1.30 GLY motCORR STED WHITE
- FWD: 0.3mm
- Coverglass: 0.14‐0.17mm
- Motorized correction collar (motCORR)
- Immersion: Glycerol
PL APO 100x/1.40 oil STED WHITE
- FWD: 0.13mm
- Coverglass: 0.17mm
- Immersion: oil
Fluorescence Cubes:
15525338 Filter cube LED 405
- Excitation: 405/60
- Emission: 470/40
15525314 Filter Cube GFP
- Excitation: BP 470/40
- Emission: BP 525/50
15525303 Filter Cube RHOD LP
- Excitation: BP 540/45
- Emission: LP 590
Location:
- Located in Bellini Building, Room 151.
Initial training sessions usually last about 3 hours and include the hourly microscope fee plus the technical support fee.
IMPORTANT: ALL training and technical support is by appointment ONLY and will depend on the availability of the ABIF staff.