Du-145 cells are a human prostate cancer cell line commonly used in research to study prostate cancer biology and drug development. These cells are androgen-insensitive and are a valuable model for investigating the mechanisms of castration-resistant prostate cancer., These cells are provided as a fraction of a T-75 flask. They are typically cultured in a standard cell culture medium supplemented with serum and antibiotics. They are frequently used in studies of cell signaling, apoptosis, and drug response.