The Agilent 2100 Expert Software User Guide is the official and comprehensive manual for operating the Agilent 2100 expert software, which serves as the control and data analysis platform for the Agilent 2100 Bioanalyzer instrument. This guide provides detailed instructions and insights for both novice and advanced users to effectively utilize the system. This software is the singular solution for managing all Bioanalyzer assays, encompassing instrument control, data acquisition, interpretation, and presentation for electrophoretic and flow cytometric analyses of DNA, RNA, proteins, and cells. It integrates essential diagnostic functions to ensure reliable operation and accurate results. Key functionalities highlighted in the guide include powerful data evaluation tools, flexible options for generating result tables and graphics, and full support for all Bioanalyzer assays. A notable feature is the unique RNA Integrity Number (RIN) algorithm, which provides an unbiased, industry-standard assessment of total RNA integrity, now also supporting plant RNA analysis. The manual details the intuitive user interface for setting up samples, selecting methods, and initiating runs. It also explains various data visualization options, such as gel-like formats and electropherograms, and outlines the multiple exportable data formats (e.g., AIA, XML, CSV, PDF, JPG) for seamless integration into presentations or Laboratory Information Management Systems (LIMS). The software is compatible with Microsoft Windows 7 (32- and 64-bit) and offers integrated diagnostic and troubleshooting capabilities.