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Digital AssetAvailable
Calibration of Wide-Field Deconvolution Microscopy for Quantitative Fluorescence Imaging
Faculty of Science
Core Facility
McGill University
Deconvolution microscopy improves fluorescence image resolution and contrast by removing out-of-focus light. This guide explains how to properly calibrate wide-field deconvolution microscopy systems, ensuring quantitative accuracy in fluorescence intensity measurements. Topics include PSF measurement, background subtraction, and algorithm selection.
Advanced BioImaging Facility
Faculty of Science
Research lab focused on advancing scientific knowledge and innovation.
CB
Claire Brown
Digital AssetAvailable
Calibration of Wide-Field Deconvolution Microscopy for Quantitative Fluorescence Imaging
Faculty of Science
Core Facility
McGill University
Deconvolution microscopy improves fluorescence image resolution and contrast by removing out-of-focus light. This guide explains how to properly calibrate wide-field deconvolution microscopy systems, ensuring quantitative accuracy in fluorescence intensity measurements. Topics include PSF measurement, background subtraction, and algorithm selection.
Advanced BioImaging Facility
Faculty of Science
Research lab focused on advancing scientific knowledge and innovation.
CB
Claire Brown
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