TIRF/Spinning Disk – Total Internal Reflection Fluorescence Microscope

Total Internal Reflection Fluorescence (TIRF) microscope for looking within ~100 nm of the microscope slide. Specifically well suited for endocytosis, exocytosis, adhesion and membrane protein/lipid studies.

Features:

• Total Internal Reflection Fluorescence (TIRF)
• Variable pinhole Spinning Disk Confocal
• Rapid simultaneous two colour imaging on EMCCD
• Environmental Control for live cell imaging

Light Sources:

• Halogen Lamp for Transmitted Light
• Wide Field Microscopy - X-Cite 120 LED (370-700nm), 100W
• Diode Laser - 488nm - 100 mW - with FLCRtm control system
• Diode Laser - 561nm - 100 mW
• Diode Laser - 642 nm - 110 mW

Objectives:

• 10X N-PLAN, NA=0.25
• 20X HC PLAN APOCHROMAT, NA=0.70,
• 63X HCX PLAN APOCHROMAT, NA=1.47, OIL, Corr, TIRF

Fluorescence Emission Filters (TIRF) - separate widefield cubes are also avaliable.

• EGFP (ET 525/50nm)
• mCherry (ET 620/60nm)
• EGFP/mCherry Dual Cube

Fluorescence Probes:

• EGFP
• mCherry
• Cy5/IR-FP

Detectors: (sCMOS are in place but can be switched for EM-CCD cameras depending on the application)

• Hamamatsu ImageEMX2 EM-CCD - 2 Cameras
• Hamamatsu C13440-20CI sCMOS - 2 Cameras

Additional Features:

• DIC Optics
• High resolution sequential multi-colour imaging

Location:

• Located in Bellini Building, Room 147.

Initial training sessions usually last about 3 hours and include the hourly microscope fee plus the technical support fee.

IMPORTANT: ALL training and technical support is by appointment ONLY and will depend on the availability of the ABIF staff.