This digital resource is a workflow protocol specifically tailored for automated immunohistochemistry (IHC) using the Tecan GenePaint system. The Tecan GenePaint is an automated slide processing system designed to streamline and standardize complex staining procedures, significantly improving throughput and reproducibility in IHC applications. This document outlines the precise steps for conducting IHC experiments on this platform. The workflow details the automated application of reagents, incubation times, and washing steps for antibody-based detection of specific antigens in tissue sections. It covers critical aspects such as antigen retrieval, blocking non-specific binding, primary and secondary antibody incubations, and chromogenic or fluorescent detection. The automation ensures consistent staining across large batches of slides, reducing variability inherent in manual procedures. Researchers in pathology, cancer biology, and preclinical research will find this protocol invaluable for high-throughput analysis of protein expression in tissue samples. The standardized and automated nature of the GenePaint system, guided by this workflow, is particularly advantageous for biomarker discovery, drug efficacy studies, and diagnostic assay development, where precision and efficiency are paramount. As a proprietary workflow optimized for the Tecan GenePaint system, it guarantees seamless integration and optimal performance. It serves as a comprehensive guide for laboratory personnel, enabling them to leverage the full capabilities of the automated system for reliable and reproducible immunohistochemistry results.