The Seahorse XFe24 Analyzer is a cellular bioenergetic analysis system that measures the rate of oxygen consumption (OCR) and extracellular acidification (ECAR) in live cells in a 24-well format. These measurements provide real-time insights into mitochondrial respiration and glycolysis, respectively. The instrument uses transient micro-chamber technology and allows for the perturbation of cellular systems by adding nutrients, drugs, or mitotoxins to observe changes in metabolic rates. It is widely used in cancer research, metabolic disease studies, and drug discovery to understand cellular metabolism and function.