The Molecular Imaging Platform is one of the Centre for Translational Biology (CTB) Technology Platforms at the Research Institute of the McGill University Health Centre (RI-MUHC). The state-of-the-art light microscopy facility specializes in cell, tissue and intravital imaging. The platform houses multiple diversified and sophisticated systems, including epifluorescent microscopes for basic applications and live cell imaging (Ca2+ and ratiometric imaging); confocal microscopes for 2D and 3D imaging; fluorescence correlation spectroscopy (FCS); super-resolution imaging (SIM, PALM and dSTORM); and live cell imaging with photo manipulation. The platform has the first multi-photon microscope with dual IR-OPO lasers for intravital imaging in Canada. Other instruments include high-content screening/imaging (HCS/HCI) microscopes for medium-size screening and imaging as well as plate readers for multimodality applications (label-free, absorbance, bioluminescence and fluorescence).