Polarized human nasal epithelial cells are primary cells cultured on porous supports (e.g., Transwells) at an air-liquid interface (ALI) to induce differentiation and establish a polarized epithelium. This culture method allows the cells to form tight junctions and develop apical-basolateral polarity, mimicking the in vivo nasal mucosa. These cells are highly valuable for studying barrier function, mucociliary clearance, and immune responses in the upper airway. They are particularly useful for research into allergic rhinitis, chronic rhinosinusitis, and the effects of environmental allergens or pathogens on nasal epithelial function.