The LI-COR Odyssey M Imaging System is a cutting-edge, multimodal imager designed for diverse applications in life science research. It functions as a comprehensive Western blot imager, plate reader, slide scanner, and luminescence imager, providing unparalleled versatility for laboratory workflows. This system is engineered to streamline data acquisition and analysis, allowing researchers to explore a wide array of assays on a single platform. Technically, the Odyssey M features a sophisticated sCMOS image sensor, delivering an industry-leading 5-micron resolution for exceptional detail, particularly in tissue section imaging. It boasts a dynamic range of over 6 logs, effectively eliminating imager saturation and ensuring accurate quantification across a broad range of signal intensities. The system offers 19 imaging channels, supporting near-infrared (NIR) and visible fluorescence, bio- and chemiluminescence, and RGB true color imaging. Its innovative line-scanning technology enables rapid data acquisition, capturing comprehensive data in minutes rather than hours. The image field size is 25 cm W × 18 cm D, with a dedicated scan area for chemiluminescence of 15 cm W × 11 cm D. The instrument dimensions are 61 cm W × 76 cm D × 38 cm H, and it weighs 55 kg with the chemiluminescence module. The Odyssey M supports an extensive range of applications, including quantitative Western blots, In-Cell Western™ assays, cell analysis, protein estimation, and high-resolution slide imaging for rapid tissue section triage. Other key applications include EMSA/Gel Shift Assay, Glycoprotein Staining, Nucleic Acid Gel Imaging, Fluorescent Gel Staining, Chemiluminescent Western Blots, Gene Knockdown and Knockout studies, Protein Arrays, ELISA, and On-Cell Western Assays. Its broad application versatility allows researchers to address complex biological questions that would typically require multiple instruments. Additional features of the Odyssey M include an onboard high-performance chemiluminescence module, eliminating the need for separate optical modules. All 19 channels are integrated and calibrated for consistent performance, and the system requires no assembly. It is compatible with LI-COR Acquisition Software and Empiria Studio® Software, which provide guided workflows for reliable and publishable results. The Odyssey M technology has been cited in over 75,000 peer-reviewed publications, underscoring its reliability and widespread adoption in the scientific community.

Optical System

1. Image Field Size: 25 cm W × 18 cm D (9.8” W × 7.1” D)
2. Scan Area of Chemi Region: 15 cm W × 11 cm D (5.9” W × 4.3” D)
3. Resolution: 5, 10, 20, 50, or 100 µm
4. Dynamic Range*: >6 logs for chemiluminescence (optional) and fluorescence
5. Detectors
6. sCMOS image sensor
7. CCD Sensor for chemiluminescence
8. Laser Lifetime
9. 685 nm and 785 nm: 20,000 hours
10. 488 nm and 520 nm: 40,000 hours
11. Class 1 Laser Product
12. Light Sources
13. RGB LED (trans-illumination)
14. RGB LED (reflective illumination)
15. Solid-state diode laser at 488 nm
16. Solid-state diode laser at 520 nm
17. Solid-state diode laser at 685 nm
18. Solid-state diode laser at 785 nm
19. Focusing
20. Microscope is adjustable -1 to 5 mm above the
21. scan bed to obtain best signal-to-noise ratio.

Size and Weight

1. Dimensions (Instrument Only)
2. 61 cm W × 76 cm D × 38 cm H
3. (24” W × 30” D × 15” H)
4. Height with lid fully open: 71 cm (28")
5. Weight
6. 55 kg (121 lbs) with chemiluminescence module
7. 52 kg (115 lbs) without chemiluminescence module