This offering provides highly-differentiated Human Bronchial Epithelial (HBE) cells pre-cultured on porous filters, such as Transwells and Snapwells. These cells are grown at an air-liquid interface (ALI) to promote differentiation into a pseudostratified mucociliary epithelium, closely mimicking the in vivo airway environment. This differentiation is characterized by the formation of cilia, mucin production, and the establishment of robust transepithelial electrical resistance (TEER) levels, which are critical for barrier function studies. These differentiated cultures are ideal for advanced studies in airway physiology, drug transport, toxicology, and host-pathogen interactions, offering a more physiologically relevant model than traditional 2D cultures. The use of porous filters allows for apical and basolateral access, facilitating studies on polarized secretion, absorption, and drug efficacy in a controlled environment.