Primary Human Bronchial Epithelial (HBE) cells are provided cultured on plastic flasks, specifically T25 flasks. These cells are suitable for initial expansion and basic cell culture applications before further differentiation or experimental use. HBE cells are crucial for research into respiratory diseases, offering a more physiologically relevant model than immortalized cell lines due to their ability to retain genetic variation and differentiate. They are typically cultured in specialized media like Bronchial Epithelial Cell Growth Medium (BEGM) to support their proliferation and maintenance in a serum-free environment. These cells are valuable for a wide range of in vitro studies, including cell proliferation assays, gene expression analysis, and preliminary drug screening, providing a foundational resource for respiratory disease investigators.