Nuclear Magnetic Resonance (NMR) spectroscopy at 600 MHz offers enhanced sensitivity and resolution compared to lower field strengths, providing more detailed structural and quantitative information about metabolites. It is a non-destructive analytical technique useful for non-targeted metabolomics and metabolic flux analysis. It detects metabolites based on the magnetic properties of atomic nuclei, enabling comprehensive analysis of complex biological samples. NMR is crucial for identifying and quantifying a wide range of compounds, including amino acids, nucleotides, and other small molecules, and is often used for studying metabolic pathways and disease mechanisms.