The Leica SP8 is a point-scanning confocal system mounted on a fully motorized Leica DMI6000B inverted microscope. It is equipped with a "SuperZ" Galvo stage for precise focusing and z-stack acquisition. The system includes four spectral fluorescence detectors—three photomultiplier tubes (PMTs) and one high-sensitivity Hybrid Detector (HyD)—along with a transmitted light detector (PMT).

McGill University

Objectives:

• 10x/0.4 Dry: HC PL APO 10x/0.40 CS
• 20x/0.7 Dry: HC PL APO 20x/0.70 CS
• 40x/0.85 Dry: HCX PL APO 40x/0.85 CORR CS
• 40x/1.1 Water: HC PL APO 40x/1.10 W CORR CS2
• 40x/1.3 Oil: HC PL APO 40x/1.30 Oil CS2
• 63x/1.4 Oil: HC PL APO 63x/1.40 Oil CS2 McGill University

Laser Lines:

• 405 nm Diode (50 mW): Excitation for DAPI
• 448 nm Diode (40 mW): Excitation for CFP
• 488 nm Diode (20 mW): Excitation for AF488, GFP, YFP
• 552 nm Diode (20 mW): Excitation for TRITC, Cy3, Texas Red, RFP
• 638 nm Diode (30 mW): Excitation for AF633, Cy5, FarRedFP McGill University

Features:

• Spectral Detection: Tunable emission detection without the need for emission filters, allowing flexibility in fluorophore selection.
• Wide-Field Fluorescence: Equipped with a Leica EL6000 metal-halide lamp and filter sets for DAPI, GFP, and RFP.
• Differential Interference Contrast (DIC): Available for high-contrast imaging of transparent specimens.

This system is ideal for high-resolution imaging applications, including multi-color fluorescence, live-cell imaging, and spectral unmixing. It is located within the Cell Imaging and Analysis Network (CIAN) at McGill University.

Initial training sessions usually last about 3 hours and include the hourly microscope fee plus the technical support fee.

IMPORTANT: ALL training and technical support is by appointment ONLY and will depend on the availability of the ABIF staff.