ImageXpressMicro from Molecular Devices is a fully-integrated hardware and software system for automated acquisition and analysis of images for high throughput cell-based screening (High-Content Screening, HCS) or high content analysis (HCA) for basic research. For more details please see the datasheet and short description.

Key Features:

• Environmental Control: Maintains precise temperature, CO₂, and humidity levels, facilitating extended live-cell imaging experiments.
• Robotic Fluidics: Enables real-time kinetic studies through automated reagent addition, allowing for dynamic monitoring of cellular responses.
• Plate Loading Robot: Supports unattended operation by automating the handling of multi-well plates, increasing throughput and efficiency.

Optics:

• Nikon Objectives:
• 4x S Fluor, NA 0.20
• 10x Plan Fluor, NA 0.30
• 20x Plan Fluor ELWD, NA 0.45
• 40x Plan Fluor ELWD, NA 0.60
• 60x Plan Fluor, NA 0.85
• 20x S Fluor, NA 0.75
• 40x S Fluor, NA 0.90
• Chroma Filter Sets:
• DAPI: Excitation 350/50 nm; Emission 460/50 nm
• eGFP/FITC/AF488: Excitation 470/40 nm; Emission 525/50 nm
• TRITC/Cy3: Excitation 545/30 nm; Emission 620/60 nm
• Texas Red/AF594: Excitation 560/40 nm; Emission 630/75 nm
• Cy5/AF647: Excitation 620/60 nm; Emission 700/75 nm
• CFP/Cyan: Excitation 436/20 nm; Emission 480/40 nm
• YFP/Yellow: Excitation 500/20 nm; Emission 535/30 nm
• Fura-2: Excitation 340/26 nm & 380/20 nm; Emission 510/80 nm
• BCECF: Excitation 440/20 nm & 495/10 nm; Emission 535/25 nm
• CFP/YFP FRET: Excitation 436/20 nm; Emission 535/30 nm

This system is located in the Bellini Building, Room 151.

Initial training sessions usually last about 3 hours and include the hourly microscope fee plus the technical support fee.

IMPORTANT: ALL training and technical support is by appointment ONLY and will depend on the availability of the ABIF staff.