Nikon CSUX-1 Disk intra-vital Confocal Microscope (in a biosecurity level 2 environment)

Applications: 4D Confocal and Multiphoton Imaging, Spectral imaging, FLIM, FRET, FRAP modules, SHG

Excitation:

- Four solid state laser lines: 448 nm, 488 nm, 552 nm,

638 nm

- One tunable IR two-photons laser: 680-1080 nm

Detection:

- Two internal HyDs (high sensitivity detectors)

- Two internal PMTs

- One transmitted light PMT

- Two reflected light HyD

- One brightfield detector (Dodt gradient)

Objectives: 4x to 63x (including air, oil and multi-immersion)

Environmental control: CO2, temperature, humidity.

Image processing and analysis: Leica LAS X and Bitplane Imaris 9.1.2

The system is capable of performing FRAP (Fluorescence Recovery After Photobleaching), Perfusion system for live tissue imaging and stimulation experiments.