Cell sorting
Sorting on the core's two BD FACSAria Fusions uses five lasers (UV 355 nm, violet 405 nm, blue 488 nm, yellow-green 561 nm and red 640 nm) and up to 18 fluorescent parameters. Sort on functional markers too, such as phospho-flow, cell cycle and mitochondrial assays.
Single cells go into 96-well plates for cloning or downstream sequencing. Collect into 1.5 ml Eppendorf tubes, 5 and 15 ml Falcon tubes, any well plate (12, 24, 48, 96 or 384), Terasaki plates or standard and frosted-end slides. Aseptic sorting is available.
You provide
- Cells or particles
- Panel, protocol or earlier data
Request this service
Flow Cytometry Core replies with scope and price.
Your details
Your lab
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Join your lab to use its details for future bookings and requests.
Nothing is booked or charged until you accept a quote.
You receive
- Your cells sorted on up to 18 colors
- Collected in tubes, plates of 12 to 384 wells, or on slides
- Single cells per well, for cloning or sequencing
How it goes
You ask
Answer a few questions about your samples.
They scope it
The facility confirms it can be done and may ask for a detail or two.
You accept a quote
Price and timing, agreed before any work starts.
Results come back
Files arrive in your requests, with a note from the facility.
Where it's been used
Sorting of CD103+, Tfh-like, CD103- and gamma delta T cells from blood and rectal biopsies of people on ART to measure HIV DNA and RNA in each.
Shared Mechanisms Govern HIV Transcriptional Suppression in Circulating CD103+ and Gut CD4+ T Cells
Yukl, Khan, Chen, et al. · Journal of Virology · 2020
Sorting of CD8+CD28+ and CD8+CD28- T cells from blood of donors aged 50 or older, to study SIRT1 loss through autophagy in aging T cells.
SIRT1 is downregulated by autophagy in senescence and ageing
Xu, Wang, Fozouni, et al. · Nature Cell Biology · 2020
Sorting of mCherry+ nuclei from human neurons transplanted into mouse hippocampi, for single-nucleus RNA-seq in a study of apoE4 toxicity.
In Vivo Chimeric Alzheimer’s Disease Modeling of Apolipoprotein E4 Toxicity in Human Neurons
Najm, Zalocusky, Zilberter, et al. · Cell Reports · 2020
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