Tissue clearing and light-sheet imaging
The core's light-sheet work covers tissue clearing and volumetric imaging on the UltraMicroscope Blaze, for samples from organoids to a whole adult mouse. The volumes can then be analyzed in Imaris on the Mammoth workstation.
You provide
- Tissue or samplesOrganoids to a whole adult mouse, fixed or frozen
- Protocol, sample list or earlier images
Request this service
Histology and Light Microscopy Core replies with scope and price.
Your details
Your lab
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Nothing is booked or charged until you accept a quote.
You receive
- Your sample imaged in 3D on the light sheet
- Image volumes ready for analysis in Imaris
How it goes
You ask
Answer a few questions about your samples.
They scope it
The facility confirms it can be done and may ask for a detail or two.
You accept a quote
Price and timing, agreed before any work starts.
Results come back
Files arrive in your requests, with a note from the facility.
Where it's been used
Light-sheet imaging of intact cardiac microtissues made from stem cells, to map each cell's position and calcium activity in 3D.
Single-Cell Determination of Cardiac Microtissue Structure and Function Using Light Sheet Microscopy
Turaga, Matthys, Hookway, et al. · Tissue Engineering Part C: Methods · 2020
Clearing and light-sheet imaging of human fetal genitalia, to follow their development from the indifferent stage to male and female.
Three-Dimensional Imaging of the Developing Human Fetal Urogenital-Genital Tract: Indifferent Stage to Male and Female Differentiation
Isaacson, Shen, Overland, et al. · Differentiation; research in biological diversity · 2018
Light-sheet imaging of a cleared, immunostained 11-week human fetal kidney, to show ureteric bud branching in 3D.
Lightsheet Fluorescence Microscopy of Branching Human Fetal Kidney
Isaacson, Shen, McCreedy, et al. · Kidney International · 2018
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