Nuclear Magnetic Resonance (NMR) spectroscopy at 500 MHz provides detailed structural and quantitative information about metabolites. It is a non-destructive analytical technique particularly useful for non-targeted metabolomics and metabolic flux analysis. It detects metabolites based on the magnetic properties of atomic nuclei, offering high resolution and sensitivity for complex biological samples. NMR is crucial for identifying and quantifying a wide range of compounds, including amino acids, nucleotides, and other small molecules, and is often used for studying metabolic pathways and disease mechanisms.