Fully automated high content microscope for fluorescence and brightfield imaging of microplates (6 to 1536 well) and standard slides. Features include 5x-60x objectives (air and water immersion), Brightfield, Digital Phase Contrast, Widefield, and confocal modes, five laser lines (375-640nm), four sCMOS cameras, fast frame-rate imaging (up to 105 fps), internal liquid handling for kinetics (e.g., Calcium imaging), and optional environmental control. It is part of a station connected to a plate handler, liquid handler, plate washer, incubator, and bar scanner.