Leica SP8 Laser Scanning Confocal and Multiphoton Microscope

Applications: Intravital Imaging, 4D Confocal, Fast Kinetics, Widefield Fluorescence, Brightfield

Excitation:

- Four lasers lines for confocal imaging: 405nm, 488nm, 561nm, 640nm.

- Four LED lines for widefield fluorescence: 385nm, 488nm, 561nm, 621nm.

- Brightfield illumination.

Emission:

- Four single chanel emission filters: 455/50nm, 525/36nm, 605/52nm, 705/72nm.

- One quad chanels filter for high speed acquisition: DAPI/FITC/TRITC/CY5.

Detection:

- Confocal: Photometrics Prime BSI Express sCMOS Camera. 2048x2048, 95fps@11-bits/43fps@16-bits.

- Widefield and Brigthfield: Nikon Digital Sight 10, Color and Monochrome mode, 25 mm FOV, 23.9 megapixel.

Objectives: 10X/0.45 air, 20X/0.75 air, 16X/0.8 water dipping, 20X/1.0 water, 60X/1.42 oil.

Located in a biosafety level 2 room that include a BSC.

Image processing and analysis: Nikon imaging software (NIS) workstation and Bitplane Imaris 9.1.2