iPS cell lines
The core distributes human iPS cell lines: WTC11, WTC10 and WTB; CRISPRi Gen1C, Gen2C and Gen1B; CRISPRn Gen1C; WTC11-GCaMP6f; and WTC NGN2. The Conklin Lab website has more on each line.
Investigators outside Gladstone need a material transfer agreement for the lines.
You provide
- Protocol or earlier data
Request this service
Stem Cell Core replies with scope and price.
Your details
Your lab
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Nothing is booked or charged until you accept a quote.
You receive
- The iPS cell line you choose from the core's list
- Outside Gladstone, the line comes under a material transfer agreement
How it goes
You ask
Answer a few questions about your samples.
They scope it
The facility confirms it can be done and may ask for a detail or two.
You accept a quote
Price and timing, agreed before any work starts.
Results come back
Files arrive in your requests, with a note from the facility.
Where it's been used
The core supplied the WTC11 iPS cell line, used to engineer knockout lines and make cardiac progenitors in a search for causes of congenital heart disease.
Transcription Factor Protein Interactomes Reveal Genetic Determinants in Heart Disease
González-Terán, Pittman, Felix, et al. · Cell · 2022
The CRISPRi iPS cell line built on WTC11, used to switch off genes in a subset of cells and study how stem cell colonies pattern themselves.
Spatiotemporal mosaic self-patterning of pluripotent stem cells using CRISPR interference
Libby, Joy, So, et al. · eLife · 2018
Origin of the CRISPRi and CRISPRn iPS cell lines the core distributes, built in WTC and WTB cells for inducible, reversible gene silencing.
CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs
Mandegar, Huebsch, Frolov, et al. · Cell Stem Cell · 2016
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