A high-parameter spectral flow cytometer designed for comprehensive cell analysis with high sensitivity to detect dim and rare populations. It can be configured with up to 7 lasers and 186 detectors, allowing researchers to perform experiments with 44 colors or more. The system uses spectral technology to generate accurate data, enabling the use of spectrally adjacent fluorochromes and fluorescent proteins. It features an advanced AutoSampler with active agitation and cooling for walkaway operation, and intuitive software with automated workflows for quality control and experiment optimization. It collects continuous light from 360 nm to 920 nm, providing more data than traditional cytometers.