This offering includes specialized cell culture media, specifically Bronchial Epithelial Cell Growth Medium (BEGM) for culture on plastic and Air-Liquid Interface (ALI) medium for culture on porous filters. BEGM is designed to support the growth and expansion of primary bronchial epithelial cells on collagen-coated plastic dishes, providing essential nutrients and growth factors for cell proliferation in serum-free conditions. ALI medium, a mixture often containing DMEM-H and LHC basal medium with specific additives, is formulated to promote the differentiation of HBE cells into a pseudostratified mucociliary epithelium when cultured on porous supports at the air-liquid interface. These specialized media are critical for maintaining the physiological relevance and functionality of primary airway epithelial cell cultures, enabling researchers to accurately model respiratory diseases and test therapeutic interventions.